Coding

Part:BBa_K2372002:Design

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


Beta-lactam-synthetase wildtype


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 9
    Illegal AgeI site found at 199
    Illegal AgeI site found at 379
    Illegal AgeI site found at 544
    Illegal AgeI site found at 1036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 631
    Illegal BsaI site found at 1224


Design Notes

Like all Streptomyces genes BLS is GC-rich.


Source

Streptomyces clavuligerus

References